CEE Faculty Profile

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Jim C. Spain

Professor

Articles

  1. Kurt, Z., K. Shin, and J.C. Spain. 2012. Biodegradation of chlorobenzene and nitrobenzene at the oxic/anoxic interface between sediment and water. Environ. Sci. Technol. 46:11829-11835.
  2. Parks, S.T., Y. Qu, and J.C. Spain. 2013. Identity and distribution of denA encoding 4-hydroxy-3-nitrophenylacetate denitrase, an FAD-dependent monooxygenase that catalyzes the key step involved in 3-nitrotyosine biodegradation in Variovorax sp. JS669. Microbiology. In revision.
  3. Pati, S.G., K. Shin, M. Skarpeli-Liati, J. Bolotin, S.N. Eustis, J.C. Spain, and T.B. Hofstetter. 2012. Carbon and nitrogen isotope effects associated with the dioxygenation of aniline and diphenylamine. Environ. Sci. Technol. 46:11844-11853.
  4. Francis, K., S. Nishino, J.C. Spain and G. Gadda. 2012. A novel activity for fungal nitronate monooxygenase: detoxification of the metabolic inhibitor propionate-3-nitronate. Arch. Biochem. Biophys. 521:84-89.
  5. Husserl, J., J.B. Hughes, and J.C. Spain. 2012. Key enzymes enable growth of Arthrobacter strain JBH1 on nitroglycerin as the sole source of carbon and nitrogen. Appl. Environ. Microbiol. 78: 3649-3655.
  6. Coleman, N.V., N.L. Wilson, K. Barry, T.S. Brettin, D.C. Bruce, A. Copeland, E. Dalin, J.C. Detter, T.G. del Rio, L.A. Goodwin, N.M. Hammon, S. Han, L.J. Hauser, S. Israni, E. Kim, N. Kyrpides, M.L. Land, A. Lapidus, F. W. Larimer, S. Lucas, S. Pitluck, P. Richardson, J. Schmutz, R. Tapia, S.Thompson, H.N. Tice, J.C. Spain, J.G. Gossett, T.E. Mattes. 2011. Genome sequence of the ethene-and vinyl chloride-oxidizing actinomycete Nocardioides sp. strain JS614. J. Bacteriol. 193:3399.
  7. Hoffstetter, T.B, J. Bolotin, M. Skarpeli-Liati, J. Wijker, Z. Kurt, S.F. Nishino and J.C. Spain. 2011. Tracking transformation processes of organic micropollutants in aquatic environments using multi-element isotope fractionation analysis. Appl. Geochem. 26S:334-336.
  8. Pumphrey, G.M., A. Ranchou-Peyruse, and J.C. Spain. Cultivation-independent detection of autotrophic hydrogen-oxidizing bacteria by DNA stable isotope probing. 2011. Appl. Environ. Microbiol.77:4931-4938.
  9. Schroeder, S., A. Ranchou-Peyruse, M. Ranchou- Peyruse, and J.C. Spain. 2011. Reverse-transcriptase quantitative PCR method to detect interspecies hydrogen exchange by Alcaligenes hydrogenophilus, an aerobic hydrogen-oxidising bacterium. Arch. Microbiol. 193:687-692.
  10. Qu, Y., and J.C. Spain. 2011. Molecular and biochemical characterization of the 5-nitroanthranilic acid degradation pathway in a Bradyrhizobium. J. Bacteriol. 193:3057-3063.
  11. Parry, R., S. Nishino, and J.C. Spain. 2011. Naturally- occuring nitro compounds. Nat. Prod. Reports. 28:152-167.
  12. Qu, Y. and J.C. Spain. 2011. Catabolic pathway for 2-nitroimidazole involves a novel nitrohydrolase that also confers drug resistance. Environ. Microbiol. 13:1010-1017.
  13. Han, J., H.-K. Choi, S.-W. Lee, P.M. Orwin, J. Kim, S.L. LaRoe, T. Kim, J. O’Neil, J.R. Leadbetter, S.Y. Lee, C.-G. Hur, J.C. Spain, G. Ovchinnikova, L. Goodwin, and C. Han. 2011. Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110. J. Bacteriol. 193:1183-1190.
  14. Nishino, S.F, K.A Shin, R. Payne, and J.C. Spain. 2010. Growth of bacteria on 3-nitropropionic acid as a sole carbon, nitrogen and energy source. Appl. Environ. Microbiol. 76:3590-3598.
  15. Qu, Y. and J.C. Spain. 2010. Biodegradation of 5-nitroanthranilic acid by Bradyrhizobium sp. Strain JS329. Appl. Environ. Microbiol. 76:1417-1422.
  16. Husserl, J. J.C. Spain, and J.B. Hughes. 2010. Growth of Arthrobacter sp. Strain JBH1 on nitroglycerin as the sole source of carbon and nitrogen. Appl. Environ. Microbiol. 76:1689-1691.
  17. Jennings, L.K., M.M.G. Chartrand, G. Lacrampe-Couloume, B.S. Lollar, J.C. Spain, and J.M. Gossett. 2009. Proteomic and transcriptomic analyses reveal genes upregulated by cis-dichloroethene in Polaromonas JS666. Appl. Environ. Microbiol. 75:3733-3744.
  18. Shin, K.E, and J.C. Spain. 2009. Biodegradation of diphenylamine by Burkholderia sp. Strain JS667: pathway and evolutionary implications. Appl. Environ. Microbiol. 75:2694-2704.
  19. Rankin, L.D., D.M. Bodenmiller, J.D. Partridge, S.F. Nishino, J.C. Spain, and S. Spiro. 2008. Escherichia coli NsrR regulates a pathway for oxidation of 3-nitrotyramine to 4-hydroxy-3-nitrophenylacetate. J. Bacteriol. 190:6170-6177.
  20. Hofstetter, T.B., J.C. Spain, S.F. Nishino, J. Bolotin, and R.P. Schwarzenbach. 2008. Identifying competing aerobic nitrobenzene biodegradation pathways by compound-specific isotope analysis. Environ. Sci. Technol. 42:4764-4770.
  21. Luckarift, H.R., and J.C. Spain. 2008. Continuous-flow applications of silica-encapsulated enzymes. Biomol. Catalysis. ACS Symposium Series. 986:243-253.
  22. Li, L. T.V. Marolla, L. Nadeau, and J.C. Spain. 2007. Probing the role of promoters in zinc reduction of nitrobenzene: continuous production of hydroxylaminobenzene. Ind. Eng. Chem. Res. 46:6840-6846.
  23. Betancor, L., H.R. Luckarift, J.H Seo, O. Brand and J.C. Spain. 2007. Three dimensional immobilization of β-galactosidase on a silicon surface. Biotechnol. Bioeng. 99:261-267.
  24. Luckarift, H.R., R. Greenwald, M. Bergin, J.C. Spain, and G.R. Johnson. 2007. Biosensor system for continuous monitoring of organophosphate aerosols. Biosens. Bioelectron. 23:400-406.
  25. Luckarift, H.R., Bosung Ku, J.C. Spain, and J. Dordick. 2007. Silica-immobilized enzymes for multi-step synthesis in microfluidic devices. Biotechnol. Bioeng. 98:701-705.
  26. Poulsen, N. C. Berne, J. Spain, and N. Kroger. 2007. Silica immobilization of an enzyme via genetic engineering of the diatom, Thalassiosira pseudonana. Angewandte Chemie. 46:1843-1846.
  27. Mattes, T.E., N.V. Coleman, A.S. Chuang, A.J. Rogers, J.C. Spain, and J.M. Gossett. 2006. Mechanism controlling the extended lag period associated with vinyl chloride starvation in Nocardioides sp. strain JS614. Arch. Microbiol. 187:217-226.
  28. Betancor, L., C. Berne, Luckarift, H.R., and J.C. Spain. 2006. Coimmobilization of a redox enzyme and a cofactor regeneration system. Chem. Comm. (34):3640-3642.
  29. Berne, C., L. Betancor, H.R. Luckarift, and J.C. Spain. 2006. Application of a microfluidic reactor for screening cancer prodrug activation using silica-immobilized nitrobenzene nitroreductase. Biomacromolecules. 7:2631-2636.
  30. He, Zhongqi, R.E. Parales, J.C. Spain, and G.R. Johnson. 2007. Novel organization of catechol meta pathway genes in the nitrobenzene degrader Comamonas sp. JS765 and its evolutionary implication. J. Ind. Microbiol. Biotechnol. 34:99-104.
  31. Luckarift, H.R., G.R. Johnson, and J.C. Spain. 2006. Silica-immobilized enzyme reactors; application to cholinesterase-inhibition studies. J. Chromatogr. B. 843:310-316.
  32. Wang, D.H, J-.B. Baek, S.F. Nishino, J.C. Spain, and L-.S. Tan. 2006. Thermally reactive phenylethynyl-terminated bis(benzylester) and bis(amide) monomers based on semi-enzymatically produced 6-phenylethynyl picolinic acid. Polymer. 47:1197-1206.
  33. Luckarift, H.R., M.B. Dickerson, K.H. Sandhage, and J.C. Spain. 2006. Rapid, room temperature synthesis of anti-bacterial bio-nano composites of lysozyme with amorphous silica or titania. Small. 2:640-642.
  34. Nadeau, L.J., J.C. Spain, R. Kannan, and L-S. Tan. 2006. Conversion of 2-(4-carboxyphenyl)-6-nitrobenzothiazole to 4-(6-amino-5-hydroxybenzothiazol-2-yl)benzoic acid by a recombinant E. coli strain. Chem. Comm. (5):564-565.
  35. Nishino, S.F., and J.C. Spain. 2006. Biodegradation of 3-nitrotyrosine by Burkholderia sp. JS165 and Variovorax paradoxus JS171. Appl. Environ. Microbiol. 72:1040-1044.
  36. Daprato, R.C., C. Zhang, J.C. Spain, and J.B. Hughes. 2005. Modeling aerobic bioremediation of 2,4-dinitrotoluene in a bioslurry reactor. Environ. Eng. Sci. 22:676-688.
  37. Johnson, G.R. and J.C. Spain. 2005. Synthesis of substituted catechols using nitroarene dioxygenases. Enzyme Microb. Technol. 38:142-147.
  38. Fournier, D., S. Trott, J.C. Spain, and J. Hawari. 2005. Metabolism of the aliphatic nitramine 4-nitro-2,4-diazabutanal (NDAB) by Methylobacterium sp. strain JS178. Appl. Environ. Microbiol. 71:4199-4202.
  39. Mattes, T.E., N.V. Coleman, J.C. Spain and J.M. Gossett. 2005. Physiological and molecular genetic analyses of vinyl chloride and ethene biodegradation in Nocardioides sp. strain JS614. Arch. Microbiol. 183:95-106.
  40. Luckarift, H.R., L.J. Nadeau, and J.C. Spain. 2005. Continuous synthesis of aminophenols from nitroaromatic compounds by combination of metal and biocatalyst. Chem. Comm. (3):383-384.
  41. Luckarift, H.R., G.R. Johnson, and J.C. Spain. 2004. Biosynthesis of hydroxydiphenylacetylene by regiospecific monooxygenation. Chem. Comm. (14):2402-2403.
  42. Naik, R.R., M.M. Tomczak, H.R. Luckarift, J.C. Spain, and M.O. Stone. 2004. Entrapment of enzymes and nanoparticles using biomimetically synthesized silica. Chem. Comm. (14):1684-1685.
  43. Zhao, J.-S., J.C. Spain, S. Thiboutot, G. Ampleman, C. Greer, and J. Hawari. 2004. Phylogeny of cyclic nitramine-degrading psychrophilic bacteria in marine sediment and their potential role in the natural attenuation of explosives. FEMS Microbiol. Ecol. 49:349-357.
  44. Bhushan B., A. Halasz, J. C. Spain, and J. Hawari. 2004. Initial reaction(s) in biotransformation of CL-20 catalyzed by salicylate 1-monooxygenase from Pseudomonas sp. Appl Environ. Microbiol. 70:4040-4047.
  45. Luckarift, H.R., J.C. Spain, R.R. Naik, and M.O. Stone. 2004. Enzyme immobilization in a biomimetic support. Nature Biotechnol. 22:211-213.
  46. Fournier, D., A. Halasz, J.C. Spain, R.J. Spanggord, J.C. Bottaro, and J. Hawari. 2004. Biodegradation of the RDX ring-cleavage product, 4-nitro-2,4-diazabutanal (NDAB) by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 70:1123-1128.
  47. Fleischmann, T.J., K.C. Walker, J.C. Spain, J.B. Hughes, and A.M. Craig. 2004. Anaerobic transformation of 2,4,6-TNT by bovine ruminal microbes. Biochem. Biophys. Res. Comm. 314:957-963.
  48. Coleman, N.V. and J.C. Spain. 2003. Epoxyalkane:Coenzyme M transferase catalyzes epoxide metabolism during biodegradation of ethene and vinyl chloride in Mycobacterium strain JS60. J. Bacteriol. 185:5536-5545.
  49. Bhushan, B., L. Paquet, J.C. Spain, and J. Hawari. 2003. Biotransformation of 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) by denitrifying Pseudomonas sp. Strain FA1. Appl. Environ. Microbiol. 69:5216-5221.
  50. Fortner, J. D., C. Zhang, J. C. Spain, and J. B. Hughes. 2003. Soil column evaluation of factors controlling biodegradation of DNT in the vadose zone. Environ. Sci. Technol. 37:3382-3391.
  51. Coleman, N.V. and J.C. Spain. 2003. Distribution of the coenzyme M pathway of epoxide metabolism among ethene- and vinyl chloride-degrading mycobacteria: evidence for plasmid-mediated lateral gene transfer. Appl. Environ. Microbiol. 69:6041-6046.
  52. Zhao, J. S., J.C. Spain and J. Hawari. 2003. Phylogenetic and metabolic diversity of RDX transforming bacteria in strictly anaerobic mixed cultures enriched on RDX as nitrogen source. FEMS Microbiol. Ecol. 46:189-196.
  53. Kadiyala, V., L.J. Nadeau, and J.C. Spain. 2003. Construction of E. coli strains for conversion of nitroacetophenones to ortho-aminophenols. Appl. Environ. Microbiol. 69:6520-6526.
  54. Johnson, G.R., and J.C. Spain. 2003. Evolution of catabolic pathways for synthetic compounds: bacterial pathways for degradation of 2, 4-dinitrotoluene and nitrobenzene. Appl. Microbiol. Biotechnol. 62:110-123.
  55. Spain, J.C., S.F. Nishino, B. Witholt, L.-S. Tan, and Wouter Duetz. 2003. Production of 6-phenylacetylene picolinic acid from diphenylacetylene by a toluene-degrading Acinetobacter. Appl. Environ. Microbiol. 69:4037-4042. 86. Bhushan, B., S. Trott, J. Spain, A. Halasz, and J. Hawari. 2003.
  56. Bhushan, B., S. Trott, J. Spain, A. Halasz, and J. Hawari. 2003. Biotransformation of RDX by cytochrome P450 2B4: Insights into the mechanism of RDX biodegradation by Rhodococcus sp.strain DN22. Appl. Environ. Microbiol. 69:1347-1351.
  57. Nadeau, L.J., Z. He, and J.C. Spain. 2003. Bacterial conversion of hydroxylamino aromatic compounds by both lyase and mutase enzymes involves intramolecular transfer of hydroxyl groups. Appl. Environ. Microbiol. 69:2786-2793.
  58. Muller, T.A., C. Werlen, J.C. Spain, and J.R. van der Meer. 2003. Evolution of a chlorobenzene degradative pathway among bacteria in a contaminated groundwater mediated by a genomic island in Ralstonia. Environ. Microbiol. 5:163-173.
  59. Bhushan, B., L. Paquet, A. Halasz, J.C. Spain, and J. Hawari. 2003. Mechanism of xanthine oxidase catalyzed biotransformation of HMX under anaerobic conditions. Biochem. Biophys. Res. Comm. 306:509-515.
  60. Trott, S., S.F. Nishino, J. Hawari, and J.C. Spain. 2003. Biodegradation of the nitramine explosive, CL-20. Appl. Environ. Microbiol. 69:1871-1874.
  61. Coleman, N.V., J.C. Spain, and T. Duxbury. 2002. Evidence that RDX biodegradation by Rhodococcus strain DN22 is plasmid-borne and involves a cytochrome p-450. J. Appl. Microbiol. 93:463-472.
  62. Coleman, N.V., T.E. Mattes, J.M. Gossett, and J.C. Spain. 2002. Phylogenetic and kinetic diversity of aerobic vinyl chloride-assimilating bacteria from contaminated sites. Appl. Environ. Microbiol. 68:6162-6171.
  63. Johnson, G.R., R.K. Jain, and J.C. Spain. 2002. Origins of the 2,4-dinitrotoluene pathway. J. Bacteriol. 184:4219-4232.
  64. Bhushan, B., A. Halasz, J. Spain, S. Thiboutot, G. Ampleman, and J. Hawari. 2002. Biotransformation of hexahydro-1,2,4-trinitro-1,3,5-triazine catalyzed by a NAD(P)H:nitrate oxidoreductase from Aspergillus niger. Environ. Sci. Technol. 36:3104-3108.
  65. Coleman, N.V., T.E. Mattes, J.M. Gossett, and J.C. Spain. 2002. Biodegradation of cis-dichloroethene as sole carbon source by a b-proteobacterium. Appl. Environ. Microbiol. 68:2726-2730.
  66. Bhushan, B., A. Halasz, J.C. Spain, and J. Hawari. 2002. Diaphorase catalyzed biotransformation of RDX via N-denitration mechanism. Biochem. Biophys. Res. Com. 296:779-784.
  67. Lessner, D.J., G.R. Johnson, R.E. Parales, J.C. Spain, and D.T. Gibson. 2002. Molecular characterization and substrate specificity analysis of nitrobenzene dioxygenase from Comamonas sp. strain JS765. Appl. Environ. Microbiol. 68:634-641.
  68. Fournier, D., A. Halasz, J. Spain, P. Fiurasek, and J. Hawari. 2002. Determination of key metabolites during biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) with Rhodococcus sp. Strain DN22. Appl. Environ. Microbiol. 68:166-172.
  69. Halaz, A., J. Spain, L. Paquet, C. Beaulieu, and J. Hawari. 2002. Insights into the formation and degradation mechanisms of methylenedinitramine during the incubation of RDX with anaerobic sludge. Environ. Sci. Technol. 36:633-638.
  70. Johnson, G.R., B.F. Smets, and J.C. Spain. 2001.Oxidative transformations of aminodinitrotoluene isomers by multicomponent dioxygenases. Appl. Environ. Microbiol. 67:5460-5466.
  71. Zhang C., R.C. Daprato, S.F. Nishino, J.C. Spain, and J.B. Hughes. 2001. Remediation of dinitrotoluene contaminated soils from former ammunition plants: soil washing efficiency and effective process monitoring in bioslurry reactors. J. Haz. Mat. 87:139-154.
  72. Davis, J. K, Z. He, G. C. Paoli, L. J. Nadeau, C. C. Somerville, and J. C. Spain. 2000. Sequence analysis and initial characterization of two isozymes of hydroxylaminobenzene mutase from Pseudomonas pseudoalcaligenes JS45. Appl. Environ. Microbiol. 66:2965-2971.
  73. He, Z., L. J. Nadeau, and J. C. Spain. 2000. Characterization of hydroxylaminobenzene mutase from pNBZ139 cloned from Pseudomonas pseudoalcaligenes JS45: a highly-associated sodium-dodecyl-sulfate-stable enzyme catalyzing an intramolecular transfer of hydroxyl group. Eur. J. Biochem. 267:1110-1116.
  74. He, Z., and J. C. Spain. 2000. One-step production of picolinic acids from 2-aminophenols catalyzed by 2-aminophenol 1,6-dioxygenase. J. Ind. Microbiol. Biotechnol. 25:25-28.
  75. He, Z., and J. C. Spain. 2000. Reactions involved in the lower pathway for degradation of 4-nitrotoluene by Mycobacterium strain HL 4-NT-1. Appl. Environ. Microbiol. 66:3010-3015.
  76. Johnson, G. R., R. K. Jain, and J. C. Spain. 2000. Properties of the trihydroxytoluene oxygenase from Burkholderia cepacia R34; an extradiol dioxygenase from the 2,4-dinitrotoluene pathway. Arch. Microbiol. 173:86-90.
  77. Nishino, S. F., G. Paoli, and J. C. Spain. 2000. Aerobic degradation of dinitrotoluenes and pathway for bacterial degradation of 2,6-dinitrotoluene. Appl. Environ. Microbiol. 66:2139-2147.
  78. Zhang, C., J.B. Hughes, S.F. Nishino, J.C. Spain. 2000. Slurry-phase biological treatment of 2,4- and 2,6-dinitrotoluene: role of bioaugmentation and effects of high dinitrotoluene concentrations. Environ. Sci.Technol. 34:2810-2816.
  79. Nadeau, L. J., He Z. and J. C. Spain. 2000. Production of 2-amino-5-phenoxyphenol from 4-nitrobiphenyl ether using nitrobenzene nitroreductase and hydroxylaminobenzene mutase from Pseudomonas pseudoalcaligenes strain JS45. J. Ind. Microbiol. Biotechnol. 24:301-305.
  80. Davis, J. K., Z. He, C. C. Somerville, and J. C. Spain. 1999. Genetic and biochemical comparison of 2-aminophenol 1,6-dioxygenase of Pseudomonas pseudoalcaligenes JS45 to meta cleavage dioxygenases: divergent evolution of 2-aminophenol meta cleavage pathway. Arch. Microbiol. 172:330-339.
  81. Smets, B. F., R. G. Riefler, U. Lendenmann, and J. C. Spain. 1999. Kinetic analysis of simultaneous 2,4-dinitrotoluene (DNT) and 2,6-DNT biodegradation in an aerobic fluidized-bed biofilm reactor. Biotechnol. Bioeng. 63:642-653.
  82. He, Z., and J. C. Spain. 1999. Preparation of 2-aminomuconate from 2-aminophenol by coupled enzymatic dioxygenation and dehydrogenation reactions. J. Ind. Microbiol. Biotechnol. 23:138-142.
  83. He, Z., and J. C. Spain.1999. Comparison of the downstream pathways for degradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45 (2-aminophenol pathway) and by Comamonas sp. JS765 (catechol pathway).1999. Arch. Microbiol. 171:309-316.
  84. Schenzle, A., H. Lenke, J.C. Spain, and H.-J. Knackmuss. 1999. Chemoselective nitro group reduction and reductive dechlorination initiate degradation of 2-chloro-5-nitrophenol by Ralstonia eutropha JMP134. Appl. Environ. Microbiol. 65:2317-2323.
  85. Schenzle, A., H. Lenke, J.C. Spain, and H.-J. Knackmuss. 1999. 3-Hydroxylaminophenol mutase from Ralstonia eutropha JMP134 catalyzes a Bamberger rearrangement. J. Bacteriol. 181:1444-1450.
  86. Nadeau, L. J., G. S. Sayler, and J. C. Spain. 1999. Oxidation of 1,1,1,-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) by Alcaligenes eutrophus A5. Arch. Microbiol. 171:44-49.
  87. Nishino, S. F., J. C. Spain, H. Lenke and H.-J. Knackmuss. 1999. Mineralization of 2, 4- and 2, 6-dinitrotoluene in soil slurries. Environ. Sci. Technol. 33:1060-1064.
  88. Haigler, B.E., G.R. Johnson, W-C. Suen, and J.C. Spain. 1999. Biochemical and genetic evidence for meta-ring cleavage of 2, 4, 5-trihydroxytoluene in Burkholderia sp strain DNT. J. Bacteriol. 181:965-972.
  89. van der Meer, J.R., C. Werlen, S. Nishino, and J.C. Spain. 1998. Evolution of a pathway for chlorobenzene metabolism leads to natural attenuation in a contaminated groundwater. Appl. Environ. Microbiol. 64:4185-4193.
  90. Kadiyala, V., and J.C. Spain. 1998. A two-component monooxygenase catalyzes both the hydroxylation of p-nitrophenol and oxidative release of nitrite from 4-nitrocatechol in Bacillus sphaericus JS905. Appl. Environ. Microbiol. 64:2479-2484.
  91. Goodall, J. L., S. M. Thomas, J. C. Spain, and S. W. Peretti. 1998. Operation of mixed-culture immobilized cell reactors for the metabolism of meta- and para-nitrobenzoate by Comamonas sp. JS46 and Comamonas sp. JS47. Biotechnol. Bioeng. 59:21-27.
  92. Kadiyala, V., B. F. Smets, K. Chandran, and J. C. Spain. 1998. High affinity p-nitrophenol oxidation by Bacillus sphaericus JS905. FEMS Microbiol. Lett. 166:115-120.
  93. He, Z., J. K. Davis, and J. C. Spain. 1998. Purification, characterization, and sequence analysis of 2-aminomuconic 6-semialdehyde dehydrogenase from Pseudomonas pseudoalcaligenes JS45. J. Bacteriol. 180:4591-4595.
  94. He, Z., and J. C. Spain. 1998. A novel 2-aminomuconate deaminase in the nitrobenzene degradation pathway of Pseudomonas pseudoalcaligenes JS45. J. Bacteriol. 180:2502-2506.
  95. Spiess, T., F. Desiere, P. Fischer, J. C. Spain, H-.J. Knackmuss, and Hiltrud Lenke. 1998. A new 4-nitrotoluene degradation pathway in a Mycobacterium sp. strain. Appl. Environ. Microbiol. 64:446-452.
  96. Vorbeck, C., H. Lenke, P. Fischer, J. C. Spain, and H-.J. Knackmuss. 1998. Initial reductive reactions in aerobic microbial metabolism of 2,4,6-trinitrotoluene (TNT). Appl. Environ. Microbiol. 64:246-252.
  97. Lendenmann, U., J.C. Spain, and B.F. Smets. 1998. Simultaneous biodegradation of 2,4-dinitrotoluene and 2,6-dinitrotoluene in an aerobic fluidized bed biofilm reactor. Environ. Sci. Technol. 32:82-87.
  98. He, Z., and J.C. Spain. 1997. Studies on the catabolic pathway of degradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45: Removal of the amino group from 2-aminomuconic semialdehyde. Appl. Environ. Microbiol. 63:4839-4843.
  99. Spain, J.C. 1997. Synthetic chemicals with potential for natural attenuation. Bioremediation J. 1:1-9.
  100. Fiorella, P. D., and J.C. Spain. 1997. Transformation of 2,4,6-trinitrotoluene (TNT) by Pseudomonas pseudoalcaligenes strain JS45. Appl. Environ. Microbiol. 63:2007-2015.
  101. Shah, M.M., and J.C. Spain. 1996. Elimination of nitrite from the explosive 2,4,6-trinitrophenylmethylnitramine catalyzed by ferredoxin NADP oxidoreductase from spinach. Biochem. Biophys. Res. Commun. 220:563-568.
  102. Lendenmann, U., and J.C. Spain. 1996. 2-Aminophenol 1,6-dioxygenase: a novel aromatic ring cleavage enzyme purified from Pseudomonas pseudoalcaligenes JS45. J. Bacteriol. 178:6227-6232.
  103. Suen, W.C., B.E. Haigler, and J.C. Spain. 1996. 2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase. J. Bacteriol. 178:4926-4934.
  104. Haigler, B.E., W.C. Suen, and J.C. Spain. 1996. Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT. J. Bacteriol. 178:6019-6024.
  105. Haigler, B.E., and J.C. Spain. 1996. Degradation of nitroaromatic compounds by microbes. SIM News Feature Article. 46:59-68.
  106. Klecka, G., S. McDaniel, P. Wilson, C. Carpenter, J. Clark, A. Thomas, and J.C. Spain. 1996. Field evaluation of a granular activated carbon fluid-bed bioreactor for treatment of chlorobenzene in groundwater. Environ. Prog. 15:93-107.
  107. Somerville, C.C., S.F. Nishino, and J.C. Spain. 1995. Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes. JS45. J. Bacteriol. 177:3837-3842.
  108. Nishino, S.F., and J. C. Spain. 1995. Oxidative pathway for the biodegradation of nitrobenzene by Comamonas sp. strain JS765. Appl. Environ. Microbiol. 61:2308-2313.
  109. Nadeau, L.J., and J.C. Spain. 1995. Bacterial degradation of 3-nitrobenzoic acid. Appl. Environ. Microbiol. 61:840-843.
  110. An, D., D.T. Gibson, and J.C. Spain, 1994. Oxidative release of nitrite from 2-nitrotoluene by a three-component enzyme from Pseudomonas sp. strain JS42. J. Bacteriol. 176:7462-7467.
  111. Jain, R.K., J.H. Driesbach, and J.C. Spain, 1994. Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp. Appl. Environ. Microbiol. 60:3030-3032.
  112. Haigler, B.E., W.H. Wallace, and J.C. Spain, 1994. Biodegradation of 2-nitrotoluene by Pseudomonas sp. strain JS42. Appl. Environ. Microbiol. 60:3466-3469.
  113. Nishino, S.F., J.C. Spain, and C.A. Pettigrew, 1994. Biodegradation of chlorobenzene by indigenous bacteria. Environ. Toxicol. Chem. 13:871-877.
  114. Haigler, B.E., S.F. Nishino, and J.C. Spain, 1994. Biodegradation of 4-methyl-5-nitrocatechol by Pseudomonas sp. strain DNT. J. Bacteriol. 176:3433-3437.
  115. Nishino, S.F., and J.C. Spain, 1993. Degradation of nitrobenzene by a Pseudomonas pseudoalcaligenes. Appl. Environ. Microbiol. 59:2520-2525.
  116. Haigler, B.E., and J.C. Spain, 1993. Biodegradation of 4-nitrotoluene by Pseudomonas sp. strain 4NT. Appl. Environ. Microbiol. 59: 2239-2244.
  117. Suen, W.C., and J.C. Spain, 1993. Cloning and characterization of Pseudomonas sp. strain DNT genes for 2,4-dinitrotoluene degradation. J. Bacteriol. 175:1831-1837.
  118. Nishino, S.F., and J.C. Spain, 1993. Cell density-dependent adaptation of Pseudomonas putida to biodegradation of p-nitrophenol. Environ. Sci. Technol. 27:489-94.
  119. Haigler, B.E., C.A. Pettigrew, and J.C. Spain, 1992. Biodegradation of mixtures of substituted benzenes by Pseudomonas sp. strain JS 150. Appl. Environ. Microbiol. 58:2237-2244.
  120. Nishino, S.F., J.C. Spain, L.A. Belcher, and C.D. Litchfield, 1992. Chlorobenzene degradation by bacteria isolated from contaminated groundwater. Appl. Environ. Microbiol. 58:1719-1726.
  121. Ross, D.D., H. Mayfield, and J.C. Spain, 1992. Environmental fate and effects of jet fuel JP-8. Chemosphere. 24:219-228.
  122. Robertson, J.B., J.C. Spain, J.D. Haddock, and D.T. Gibson, 1992. Oxidation of nitrotoluenes by toluene dioxygenase: evidence for a monooxygenase reaction. Appl. Environ. Microbiol. 58:2643-2648.
  123. Spanggord, R.J., S.F. Nishino, and J.C. Spain, 1991. Biodegradation of 2,4-dinitrotoluene by a Pseudomonas sp. Appl. Environ. Microbiol. 57:3200-3205.
  124. Haigler, B.E., and J.C. Spain, 1991. Biotransformation of nitrobenzene by bacteria containing toluene degradative pathways. Appl. Environ. Microbiol. 57:3156-3162.
  125. Spain, J.C. and D.T. Gibson. 1991. Metabolic pathway for biodegradation of p-nitrophenol in Moraxella. Appl. Environ. Microbiol. 57:812-819.
  126. Pettigrew, C.A., B.E. Haigler, and J.C. Spain. 1991. Simultaneous biodegradation of toluene and chlorobenzene by Pseudomonas sp. strain JS6. Appl. Environ. Microbiol. 57:157-162.
  127. Spain, J.C., G.J. Zylstra, C.K. Blake, and D.T. Gibson. 1989. Monohydroxylation of phenol and 2,5-dichlorophenol by toluene dioxygenase in Pseudomonas putida F1. Appl. Environ. Microbiol. 55:2648-2652.
  128. Haigler, B.E. and J.C. Spain. 1989. Degradation of p-chlorotoluene by a mutant of Pseudomonas sp. strain JS6. Appl. Environ. Microbiol. 55:372-379.
  129. Spain, J.C. and D.T. Gibson. 1988. Oxidation of substituted phenols by Pseudomonas putida F1 and Pseudomonas sp. JS6. Appl. Environ. Microbiol. 54:1399-1404.
  130. Spain, J.C., D.C. Downey, and J. Milligan. 1989. Excessive bacterial decomposition of H2O2 during enhanced biodegradation. Groundwater. 27:163-167.
  131. Haigler, B.E., S.F. Nishino, and J.C. Spain. 1988. Degradation of 1,2-dichlorobenzene by a Pseudomonas sp. Appl. Environ. Microbiol.54:294-301.
  132. Spain, J.C., and S.F. Nishino. 1987. Degradation of 1,4-dichlorobenzene by a Pseudomonas sp. Appl. Environ. Microbiol. 53:1010-1019.
  133. Pritchard, P.H., C.R. Cripe, W.W. Walker, J.C. Spain, and A.W. Bourquin. 1987. Biotic and abiotic degradation rates of methyl parathion in freshwater and estuarine water and sediment. Chemosphere. 16:1509-1520.
  134. Somerville, C.C., C.A. Monti, and J.C. Spain. 1985. Modification of the 14C-most-probable-number method for use with nonpolar and volatile substrates. Appl. Environ. Microbiol. 49:711-713.
  135. Spain, J.C., and C.C. Somerville. 1985. Fate and toxicity of high density missile fuels in aquatic test systems. Chemosphere. 14:239-248.
  136. Spain, J.C., P.A. Van Veld, C.A. Monti, P.H. Pritchard, and C.R. Cripe. 1984. Comparison of p-nitrophenol degradation in field and laboratory test systems. Appl. Environ. Microbiol. 48:944-950.
  137. Van Veld, P.A., and J.C. Spain. 1983. Degradation of selected xenobiotic compounds in three types of aquatic test systems. Chemosphere. 12:1291-1305.
  138. Spain, J.C., and P.A. Van Veld. 1983. Adaptation of natural microbial communities to degrade xenobiotic compounds: effect of concentration, exposure time, inoculum, and chemical structure. Appl. Environ. Microbiol. 45:428-435.
  139. Spain, J.C., P.H. Pritchard, and A.W. Bourquin. 1980. Effects of adaptation on biodegradation rates in sediment/water cores from estuarine and freshwater environments. Appl. Environ. Microbiol. 40:726-734.
  140. Spain, J.C., O. Wyss, and D.T. Gibson. 1979. Enzymatic oxidation of p-nitrophenol. Biochem. Biophys. Res. Comm. 88:634-641.
  141. Bellion, Edward, and J.C. Spain. 1976. Distribution of isocitrate lyase serine pathway amongst one-carbon utilizing organisms. Can. J. Microbiol. 22:404-408.

Books

  1. Jennings, L.K., C.G.S. Giddings, J.M. Gossett, and J.C. Spain. 2013. Bioaugmentation for aerobic degradation of cis-1,2-dichloroethylene. In Stroo, H.F., A. Leeson, and C.H. Ward (eds). Bioaugmentation for Groundwater Remediation. SERDP ESTCP Environmental Remediation Technology. Volume 5:199-217.
  2. Nishino, S. F., and J. C. Spain. 2004. Catabolism of nitroaromatic compounds. pp. 575-608. In: J.-L. Ramos (ed.), The Pseudomonads Vol III. Biosynthesis of macromolecules and molecular metabolism. Kluwer Academic/Plenum Publishers.
  3. Ward, C.H., J.B. Hughes, G.A. Pope, M. Delshad, V. Dwaranath, J. Spain, S. Nishino, J.S. Fruchter, V.R. Vermeul, M.D. Williams, and J.E. Szecsody. 2002. In situ treatment technologies. In: D. Reible and K. Demnerova (eds.) Innovative Approaches to the On-Site Assessement and Remediation of Contaminated Sites. Kluwer Acacemic Publishers, Dordrecht, The Netherlands.
  4. Nishino, S.F. and J.C. Spain. 2001. Identification of bottlenecks to the in situ bioremediation of dinitrotoluene. In: Magar, V.S., G. Johnson, S.K. Ong, and A. Leeson (eds.) Bioremediation of Energetics, Phenolics, and Polycyclic Aromatic Hydrocarbons. Battelle Press, Columbus, OH.
  5. Nishino, S.F. and J.C. Spain. Biodegradation, transformation and bioremediation of nitroaromatic compounds. 2002. In: C.H. Hurst, G.R. Knudsen, M.J. McInerney, L.D. Stetzenbach, and M.V. Walter (eds.) Manual of Environmental Microbiology, 2nd Edition. ASM Press, Washington DC.
  6. Herrington, R.T., J. Hicks, D. Downey, J.C. Spain, S.F. Nishino, J. Gossett, and E. Becvar. 2000. Natural attenuation of chlorinated benzenes at a former disposal site. In: G.B. Wickramanayake, A.R. Gavaskar, and M.E. Kelley (eds.) Natural Attenuation Considerations and Case Studies: Remediation of Chlorinated and Recalcitrant Compounds. Battelle Press, Columbus, OH.
  7. Nishino, S. F., J. C. Spain and Z. He. 2000. Strategies for aerobic degradation of nitroaromatic compounds: process discovery to field application. In Spain, J. C., J. B. Hughes, and H. -J. Knackmuss (eds.). Biodegradation of Nitroaromatic Compounds and Explosives. Lewis Publishers, Boca Raton, FL
  8. Spain, J. and S. Nishino. 2000. Oxidative pathways for biodegradation of nitroaromatic compounds. Proceedings of the Ninth European Congress on Biotechnology. Branche Belge de la Societe de Chimie Industrielle, Brussels, Belgium.
  9. Spain, J. C., S. F. Nishino, M. R. Green, J. E. Forbert, N. A. Nogalski, R. Unterman, W. M. Riznychok, S. E. Thompson, P. M. Sleeper, and M. A. Boxwell. 1999. Field demonstration of FBR for treatment of nitrotoluenes in groundwater. In: B. C. Alleman and A. Leeson (eds.) Bioremediation of Nitroaromatic and Haloaromatic Compounds. Battelle Press, Columbus, OH.
  10. Ward, C. H., M. Alexander, J. Ryan, and J. C. Spain. 1999. Transformation. In: W. C. Anderson, R. C. Loehr, and B. P. Smith (eds), Environmental Availability in Soils. American Academy of Environmental Engineers, Wash. DC.
  11. Nishino, S. F., and J. C. Spain. 1999. Strategies for aerobic biodegradation of dinitrotoluenes. In R. Fass, .Y. Flashner, and S. Reuveny (eds.) Novel Approaches for Bioremediation of Organic Pollution. Plenum Press, New York.
  12. Nishino, S.F. and J.C. Spain, 1996. Biodegradation and transformation of nitroaromatic compounds. In C.H. Hurst, G.R. Knudsen, M.J. McInerney, L.D. Stetzenbach, and M.V. Walter (eds.) Manual of Environmental Microbiology. ASM Press, Washington, DC.
  13. Spain, J.C., 1995. Biodegradation of nitroaromatic compounds. In: L.N. Ornston, A. Ballows, and E.P. Greenberg (Eds.) Annual Reviews in Microbiology. Vol. 49:523-549. Annual Reviews Inc. Palo Alto, CA.
  14. Spain, J.C., 1995. Bacterial degradation of nitroaromatic compounds under aerobic conditions. In J.C. Spain (ed.). Biodegradation of nitroaromatic compounds. Plenum Press, New York.
  15. Spain, J.C., C.A. Pettigrew, B.E. Haigler, 1991. Biodegradation of mixed solvents by a strain of Pseudomonas. In: G.S. Sayler et al (eds). Environmental Biotechnology for Waste Treatment. Plenum Press, New York.
  16. Spain, J.C., 1990. Microbial adaptation in aquatic ecosystems. In: K.D. Racke and J.R. Coates (eds). Enhanced Biodegradation Pesticides in the Environment. American Chemical Society, Washington, D.C.
  17. Spain, J.C., 1990. Metabolic pathways for biodegradation of chlorobenzenes. In: S. Silver et al (eds). Pseudomonas: Biotransformations, Pathogenesis, and Evolving Biotechnology. American Society for Microbiology, Washington, D.C.
  18. Spain, J.C., 1988. Development of bacteria for biodegradation of chloroaromatic compounds. In: G. Lewandowski et al (eds). Biotechnology Applications in Hazardous Waste Treatment. Engineering Foundation, New York.

Proceedings

  1. Nishino, S.F., S. Craven, E. Lang, and J.C Spain. 2011. Biodegradation of m-tyrosine by rhizosphere bacteria. 111th General Meeting of the American Society for Microbiology
  2. Smitherman, C., K. Francis, S. Nishino, JC Spain, and G. Gadda. 2011. Detoxification of a lethal toxin by nitronate monooxygenase. International Symposium on Flavins and Flavoproteins, Berkeley, CA.
  3. Gadda, G., K. Francis, S. Nishino, and JC Spain. 2011. Propionate-3-nitronate oxidation by fungal nitronate monooxygenase: a 60- year mystery solved. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways.
  4. Wijker, R.S Zohre Kurt, Jim C. Spain, Jakov Bolotin, and Thomas B. Hofstetter. 2011. Enzyme kinetics of flavin-dependent monooxygenases modulate the observable isotope fractionation during monooxidation of 4-nitrophenol. American Chemical Society Symposium on Reaction Mechanisms in Environmental Organic Chemistry. Denver, CO.
  5. Cox, E., C. Austrins, J. Spain, K. Shin, S. Nishino, J. Gossett, C. Giddings, L. Jennings, E. Edwards, T. Johnson, M. Duhamel, and B. Sherwood-Lollar. 2010. The truth is out there: unraveling the mystery of the missing DCE, vinyl chloride, and ethane. Seventh International Conference on Remediation of Chlorinated and Recalcitrant Compounds, Monterey, CA.
  6. Sherwood-Lollar, B., J. McKelvie, M. Elsner, S. Mancini, T. Johnson, M. Simpson, A. Simpson, E. Edwards, J. Gossett, L. Jennings, J.C Spain, and E. Cox. 2010. New developments in forensic evaluation of contaminant sources and microbial degradation pathways using CSIA. Seventh International Conference on Remediation of Chlorinated and Recalcitrant Compounds, Monterey CA.
  7. Wan Johari, W.L., J.C. Spain, and J.M. Gossett. 2010. The involvement of a cyclohexanone monooxygenase gene of Polaromonas sp. JS666 during aerobic cis-dichloroethene degradation. Seventh International Conference on Remediation of Chlorinated and Recalcitrant Compounds, Monterey CA.
  8. Major, D., C. Aziz, J.M. Gossett, J.C. Spain, and B. Sherwood-Lollar. 2009. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis-1,2-DCE. Partners in Environmental Technology Technical Symposium & Workshop, SERDP/ESTCP. Washington, D.C.
  9. Cox, E., J.M. Gossett, J.C. Spain, E. Edwards, and B. Sherwood-Lollar. 2009. The truth is out there: unraveling the mystery of the missing DCE, vinyl chloride, and ethane. Partners in Environmental Technology Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  10. Hofstetter, T.B, A.E. Hartenbach, J.C. Spain, S.F. Nishino, and R.P. Schwarzenbach. 2009. Using compound-specific carbon and nitrogen isotope analysis for the assessment of nitroaromatic compound transformation. American Chemical Society National Meeting, Salt Lake City.
  11. Qu, Yi and J.C. Spain. 2009. Biodegradation of 5-nitroanthranilic acid by a Bradyrhizobium sp. Annual Meeting of the American Society for Microbiology.
  12. Shin, Kwanghee and J.C. Spain. 2009. Biodegradation of diphenylamine by Burkholderia sp, JS667. Annual Meeting of the American Society for Microbiology.
  13. Shin, Kwanghee and J.C. Spain . 2009. Biodegradation of nitrobenzene, aniline, and diphenylamine at the oxic/anoxic interface between sediment and water. RemTec Remediation Technology Summit.
  14. Cox, E., C. Austrins, J. Gossett, J. Spain, E. Edwards, and B. Sherwood-Lollar. 2008. Elucidation of the mechanisms and environmental relevance of cis-dichloroethene and vinyl chloride biodegradation. Partners in Environmental Technology Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  15. Major, D., J. Gossett, L. Jennings, C. Sausville-Giddings, S. Nishino, J. Spain, C. Aziz, and M. Watling. 2008. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis-1,2-DCE. Partners in Environmental Technology Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  16. Nishino, S.F, R.B. Payne, and J.C. Spain. 2008. Pathway for bacterial degradation of 3-nitropropionic acid and the purification and characterization of a novel denitrase. Annual Meeting of the American Society for Microbiology.
  17. Jennings, L.K., S.F. Nishino, R.B. Payne, J.C. Spain, and J.M. Gossett. 2008. Proteomic and transcriptomic analyses reveal genes upregulated by cis-dichloroethene in Polaromonas sp. JS666. Annual Meeting of the American Society for Microbiology.
  18. Shin, K. and J.C. Spain. 2007. Biodegradation of diphenylamine. Annual Meeting of the American Society for Microbiology.
  19. Seo, J.H, L. Betancor, K. S. Demirci, J.C Spain, and O. Brand. 2007. Liquid-Phase biochemical sensing with disk-type resonant microsensor. Transducers- 14th International Conference on Solid-State Sensors, Actuators, and Microsystems, Lyon, France.
  20. Kroeger, N., N.C. Poulsen, and J.C. Spain. 2007. Biogenesis of functional silica nanostructures through diatom genetic engineering. Society for Industrial Microbiology, Annual Meeting.
  21. Jennings, L. K., J. M. Gossett, and J. C. Spain. 2007. Deciphering cDCE degradation pathways in Polaromonas sp. strain JS666. Ninth International In- Situ and On-Site Bioremediation Symposium, Baltimore MD.
  22. Aziz, C.E., M. Watling, D. Major, L.K. Jennings, C.G. Sausville-Giddings, J.M. Gossett, S. Nishino and J.C. Spain. 2007. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis-1,2-DCE. Partners in Environmental Technology - Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  23. Aziz, C.E., M. Watling, D. Major, L.K. Jennings, C.G. Sausville-Giddings, J.M. Gossett, S. Nishino and J.C. Spain. 2006. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis-1,2-DCE. Partners in Environmental Technology - Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  24. H.R. Luckarift, G.R. Johnson, M.M Tomczak, R.R. Naik, and J.C. Spain. 2006. Biomimetic silica encapsulation: an efficient and versatile enzyme immobilization technique. AIChE Annual Meeting, Bionanotechnology Symposium.
  25. Goldstein, K., R. Deeb, R. O’Laskey, M.C. Cavanaugh, J. Spain, J. Hughes, F. Bogle, S. Muffler, and L. Haines. 2006. Alternative closure strategy in karst setting at VAAP. Battelle-Fifth International Conference on Remediation of Chlorinated and Recalcitrant Compounds.
  26. Wang, D.H., J.-B. Baek, S.F. Nishino, J.C. Spain and L-.S. Tan. 2006. Semi-enzymatically produced 6-phenylethynyl picolinic acid as endcapping agent: Synthesis and thermal properties of phenylethynyl-terminated bis(benzylester) and bis(amide) monomers. Annual Meeting of the American Chemical Society. Polymer Preprints 47(1), 266-267.
  27. Nadeau, L.J., J.C. Spain, R. Kannan, and L-S. Tan. 2005. Nitroreductase and mutase produce a novel aminophenolic polymer synthon. Annual Meeting of the American Society for Microbiology.
  28. Eby, D.M., and J.C. Spain. 2005. Ring fission of 3-methyl-4-nitrocatechol during 2,6-dinitrotoluene degradation is catalyzed by a novel extradiol dioxygenase. Annual Meeting of the American Society for Microbiology.
  29. Johnson, G.R., D. M. Eby, and J. C. Spain. 2005. Transformation of aminomethylnitrocatechol isomers - towards an engineered pathway for aerobic 2,4,6-trinitrotoluene degradation. Annual meeting of the American Society for Microbiology.
  30. Berne, C., and J.C. Spain. 2005. Purification and characterization of hydroxylaminobenzene mutases A and B from Pseudomonas pseudoalcaligenes JS45. Annual meeting of the American Society for Microbiology.
  31. Mattes, T.E., N.V. Coleman, J.C. Spain, and J.M. Gossett. 2005. Characterization of the extended lag period associated with vinyl chloride and ethene starvation in Nocardioides sp. strain JS614. Annual meeting of the American Society for Microbiology.
  32. Nishino, S.F., and J.C. Spain. 2004. Biodegradation of 3-nitrotyrosine by Variovorax paradoxus strain JS171 and Burkholderia sp. JS165. Annual meeting of the American Society for Microbiology.
  33. Johnson, G.R., and J.C. Spain. 2004. Synthesis of novel catechols using bacterial nitroarene dioxygenases. Annual meeting of the American Society for Microbiology.
  34. H.R. Luckarift and J.C. Spain. 2005. A microbial 'toolbox' for the biosynthesis of novel polymer synthons from diphenylacetylene. Annual meeting of the American Society for Microbiology
  35. Mattes, T.E., J.M. Gossett, N.V. Coleman, and J.C. Spain. 2004. A linear plasmid carries vinyl chloride pathway genes in Nocardioides strain JS614. Battelle International Symposium.
  36. Naik, R.R., H.R. Luckarift, J.C. Spain, and M. Stone. 2004. Biomimetic encapsulation of enzymes. Annual Meeting of the Materials Research Society.
  37. Mattes, T.E., J.M. Gossett, N.V. Coleman, and J.C. Spain. 2003. Characterization of vinyl chloride and ethane starvation physiology in VC-assimilating Nocardioides strain JS614. Battelle International Symposium on In-Situ and On-Site Bioremediation.
  38. Fleischmann, T.J., S.L. Ivey, K.C. Walker, J.C. Spain, and A.M. Craig. 2003. Anaerobic transformation of 2,4,6-TNT by bovine ruminal microbes. Annual meeting of the American Society for Microbiology.
  39. Mattes, T.E., N.V. Coleman, J.C. Spain, and J.M. Gossett. 2003. Evidence that vinyl chloride monooxygenase genes are encoded by a megaplasmid in Nocardioides strain JS614. Annual meeting of the American Society for Microbiology.
  40. Kachel, C.A, R. Giannone, R.E. Parales, G.R. Johnson, J.C. Spain, and G.J. Zylstra. 2003. Nucleotide Sequence Analysis of the Burkholderia sp. strain DNT Large catabolic plasmid for dinitrotoluene degradation. Annual meeting of the American Society for Microbiology.
  41. Nadeau, L.J., Z. He, and J.C. Spain. 2002. Intramolecular transfer of hydroxyl groups during bacterial conversion of hydroxylamino aromatic compounds by both lyase and mutase enzymes. Annual meeting of the American Society for Microbiology.
  42. Trott, S., J. Hawari, and J.C. Spain. 2002. Biodegradation of the cyclic nitramine explosive CL-20. Annual meeting of the American Society for Microbiology.
  43. Coleman, N. and J. Spain. 2001. Aerobic biodegradation of cis-dichloroethylene as sole carbon source by a beta-proteobacterium, strain JS666. ASM International Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan, Puerto Rico.
  44. Nishino, S.F., J.C. Spain, W.A. Duetz, and B. Witholt. 2001. Production of 6-phenylacetylene picolinic acid from diphenylacetylene by a toluene-degrading Acinetobacter. ASM International Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan, Puerto Rico.
  45. Paoli, G.C. and J.C. Spain. 2001. Solubilization and purification of a membrane associated hydroxylaminobenzene mutase. ASM International Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan, Puerto Rico.
  46. Johnson, G.R., B.F. Smets, and J.C. Spain. 2001. Oxidative transformation of aminodinitrotoluene isomers by multicomponent dioxygenases. ASM International Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan, Puerto Rico.
  47. DaPrato, R.C., C. Zhang, S.F. Nishino, J.C. Spain, and J.B. Hughes. 2001. Interpretation and modeling of slurry reactor performance to provide monitoring strategies for the aerobic bioremediation of dinitrotoluene contaminated soils. NATO Advanced Study Institute. Prague, Czech Republic.
  48. Zhang, C., J.B. Hughes, R. Deprato, S.F. Nishino, and J.C. Spain. 2001. Stoichiometry of dinitrotoluene mineralization in a pilot-scale slurry-phase bioreactor system. Battelle International Symposium on In-Situ and On-Site Bioremediation.
  49. S.M. Cuffin, P.M. Lafferty, P.N. Taylor, J.C. Spain, and S.F. Nishino. 2001. Bioremediation of dinitrotoluene isomers in the unsaturated/saturated zone. Battelle International Symposium on In-Situ and On-Site Bioremediation.
  50. Johnson, G.R., P.C. Gilcrease and J.C. Spain. 2000. Biocatalytic synthesis of polymer synthons using multicomponent toluene monooxygenases. Annual meeting of the American Society for Microbiology.
  51. Nishino, S.F. and J.C. Spain. 2000. In situ biodegradation of 2,4- and 2,6-dinitrotoluene. Annual Meeting American Society for Microbiology.
  52. Deeb, R. A., S. F. Nishino, J. C. Spain, H. Y. Hu, K. Scow, and L. Alvarez-Cohen. 2000. MTBE and benzene biodegradation by a bacterial isolate via two independent monooxygenase-initiated pathways. Annual Meeting American Chemical Society.
  53. J.C. Spain, S.F. Nishino, C. Zhang, and J.B. Hughes. 1999. DNT degradation in soil from Badger Army Ammunition Plant. Partners in Environmental Technology Technical Symposium and Workshop.
  54. Johnson, G.R. and J. C. Spain. 1999. Evolutionary origins of the pathway for 2,4-dinitrotoluene degradation. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives.
  55. Paoli, G.C., S. F. Nishino and J. C. Spain. 1999. Metabolism of 3-methyl-4-nitrocatechol by 2,6-dinitrotoluene degrading bacteria. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives.
  56. Nishino, S.F. and J.C. Spain. 1999. 2,4-DNT degradation in soil from Badger Army Ammunition Plant. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives.
  57. Zhang, C., S. F. Nishino, J. B. Hughes, and J. C. Spain. 1999. Aerobic biodegradation of 2,4-dinitrotoluene and 2,6-dinitrotoluene in a pilot-scale sequential slurry reactor system. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives.
  58. Nadeau, L. J., Zhongqi He, and J. C. Spain. 1999. The biosynthesis of 2-amino-5-phenoxyphenol from 4-nitrobiphenyl ether using two enzymes, a nitroreductase and hydroxylaminobenzene mutase, from Pseudomonas pseudoalcaligenes JS45. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives
  59. Deeb, R. A., J. C. Spain, and L. Alvarez-Coehn. 1999. Mineralization of benzene, toluene, ethylbenzene, m-xylene, and p-xylene by two Rhodococcus species. Annual Meeing American Society for Microbiology.
  60. He, Z., L. Nadeau, and J. C. Spain. 1999. Characterization of hydroxylaminobenzene mutase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  61. Deeb, R. A., J. C. Spain, and L. Alvarez-Coehn. 1999. Mineralization of BTEX compounds and MTBE in pollutant mixtures by enriched and pure microbial cultures. Battelle International Symposium on In-Situ and On-Site Bioremediation.
  62. Spain, J.C., and J.C. Cornette. 1998. Biotreatment of nitroaromatic compounds. US/Canada/UK TTCP Workshop on Environmental Aspects of Energetic Materials. Quebec, Canada.
  63. Spain, J.C. 1998. Biotreatment of dinitrotoluenes from munitions manufacturing sites. US/Germany Environmental Technology Data Exchange Meeting Johnson, G.R., B.E. Haigler, R.K. Jain, and J.C. Spain. 1998. Cloning and characterization of genes encoding enzymes for degradation of 2,4-dinitrotoluene from Burkholderia cepacia R34. Annual Meeting American Society for Microbiology.
  64. He, Z., J.K. Davis, and J.C. Spain. 1998. Purification, characterization, and sequence analysis of 2-aminomuconic-6-semialdehyde dehydrogenase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  65. Spain, J.C., S.F. Nishino, C. Werlen, and J.R. van der Meer. 1998. Evolution of a pathway for chlorobenzene metabolism in a contaminated ecosystem. Annual Meeting American Society for Microbiology.
  66. Nishino, S.F., and J.C. Spain. 1998. Mineralization of 2,4- and 2,6-dinitrotoluene in soil slurries by pure cultures of DNT-degrading bacteria. Annual Meeting American Society for Microbiology.
  67. Smets, B.F., J.C. Spain, and R.J. Mueller. 1998. Desorption and aerobic biodegradation of dinitrotoluenes in aged contaminated soils. Battelle Conference on Remediation of Chlorinated and Recalcitrant Compounds.
  68. Spiess, Tillman, Frank Desiere, Jim Spain, Hans-Joachim Knackmuss, and Hiltrud Lenke, 1997. Degradation of 4-nitrotoluene by a Mycobacterium sp. strain. Annual Meeting of the German Association of Microbiology-Hamburg.
  69. Davis, John K., C.C. Somerville, and J.C. Spain, 1997. Pseudomonas pseudoalcaligenes JS45 possesses two genes encoding hydroxylaminobenzene mutase. Annual Meeting American Society for Microbiology.
  70. Davis, John K., C.C. Somerville, and J.C. Spain, 1997. Cloning and characterization of the genes for 2-aminophenol 1,6-dioxygenase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  71. Lendenmann, Urs, B.F. Smets, and J.C. Spain, 1997. Biodegradation of 2,4-DNT and 2,6-DNT in a fluidized bed reactor with a mixed bacterial culture. Annual Meeting American Society for Microbiology.
  72. Venkateswarlu, K. and J.C. Spain, 1997. Nitrophenol biodegradation by Bacillus sphaericus JS905 expressing a soluble monooxygenase. Annual Meeting American Society for Microbiology.
  73. He, Z. and J.C. Spain, 1997. Final steps in the pathway for biodegradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  74. Johnson, G.R., and J.C. Spain, 1997. Phylogenetic analysis of nitrotoluene dioxygenases. Annual Meeting American Society for Microbiology.
  75. Haigler, B.E., C.C. Somerville, and J.C. Spain, 1997. Sequence analysis differences between the 2,4-dinitrotoluene degrading genes of Burkholderia sp. strain R34 and strain DNT. Annual Meeting American Society for Microbiology.
  76. Nishino, S.F., and J.C. Spain, 1997. Ring cleavage of 3-methyl-4-nitrocatechol by 2,6-dinitrotoluene degrading bacteria. Annual Meeting American Society for Microbiology.
  77. Thomas, A., G. Klecka, and J.C. Spain, 1996. Treatment of chlorobenzene contaminated groundwater using a fluidized bed bioreactor. Engineering Foundation Conference on Bioremediation of Surface and Subsurface Contamination.
  78. Lendenmann, U., and J.C. Spain, 1996. 2-Aminophenol 1,6-dioxygenase: a novel aromatic ring-cleavage enzyme purified from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  79. Davis, J.K., C.C. Somerville and J.C. Spain, 1996. Cloning of hydroxylaminobenzene mutase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  80. Nadeau, L.J. and J.C. Spain, 1996. Oxidation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p’-DDT) by Alkaligienes eutrophus A5. Annual Meeting American Society for Microbiology.
  81. Driesbach, J., S. Nilsen, C. Shelley, and J. Spain, 1996. Biodegradation of 4-halophenols by Arthrobacter sp. Annual Meeting American Society for Microbiology.
  82. Nishino, S.F., and J.C. Spain, 1996. Degradation of 2,6-dinitrotoluene by bacteria. Annual Meeting American Society for Microbiology.
  83. Somerville, C.C., and J.C. Spain, 1996. Purification of a novel nitroreductase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  84. Schenzle, A., H. Lenke, J.C. Spain, and H.-J. Knackmuss, 1996. 3-Hydroxylaminophenol mutase from Alcaligenes eutrophus JMP134 catalyzes a Bamberger-type rearrangement. Annual Meeting American Society for Microbiology.
  85. Fiorella, P.D., and J.C. Spain, 1996. Transformation of TNT by Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  86. Nishino, S.F., and J.C. Spain, 1995. The effect of 2,6-dinitrotoluene on 2,4-dinitrotoluene degradation. Annual Meeting Society for Environmental Toxicology and Chemistry.
  87. Parales, R.E., J.V. Parales, D. An, A. Kumar, S.M. Resnick, J.C. Spain, and D.T. Gibson, 1995. 2-Nitrotoluene dioxygenase from Pseudomonas sp. Strain JS42: Protein purification, Molecular Biology, and Substrate Specificity. American Society for Microbiology International Symposium on Pseudomonas.
  88. Shah, M.M., and J.C. Spain, 1995. Reduction of the explosive 2,4,6-trinitrophenylmethylnitramine (tetryl) catalyzed by oxygen sensitive nitro reductase enzymes. Nitration Symposium, National Meeting American Chemical Society.
  89. Haigler, B.E., and J.C. Spain, 1995. Purification and characterization of 4-methyl-5-nitrocatechol oxygenase from Pseudomonas sp. strain DNT. Annual Meeting American Society for Microbiology.
  90. Nishino, S.F., and J.C. Spain, 1995. Degradation of mixtures of nitroaromatic compounds by a gram positive bacterium. Annual Meeting American Society for Microbiology.
  91. Vorbeck, C., H. Lenke, J.C. Spain, and H.-J. Knackmuss, 1994. Initial steps in aerobic metabolism of 2,4,6-trinitrotoluene (TNT) by a Mycobacterium sp. Annual Meeting, American Society for Microbiology.
  92. An, D., D.T. Gibson, W. Wallace, and J.C. Spain, 1994. Oxidative release of nitrite from 2-nitrotoluene by a three-component enzyme system from Pseudomonas sp. strain 2NT. Annual Meeting, American Society for Microbiology.
  93. Klecka, G., S. McDaniel, P. Wilson, C. Carpenter, J. Clark, A. Thomas, and J.C. Spain, 1994. Bioreactor treatment of chlorobenzene in contaminated groundwater: results of a site demonstration. Annual Meeting, American Institute of Chemical Engineers
  94. Nadeau, L.J., and J.C. Spain, 1994. The bacterial degradation of m-nitrobenzoic acid. Annual Meeting, American Society for Microbiology.
  95. Nishino, S.F., and J.C. Spain, 1994. Oxidative pathway for the degradation of nitrobenzene by a gram-positive bacterium. Annual Meeting, American Society for Microbiology.
  96. Suen, W.C., B.E. Haigler, and J.C. Spain, 1994. 2,4-Dinitrotoluene dioxygenase genes from Pseudomonas sp. strain DNT. Annual Meeting, American Society for Microbiology.
  97. Somerville, C.C., S.F. Nishino, and J.C. Spain, 1994. Purification and characterization of nitrobenzene reductase from Pseudomonas pseudoalcaligenes, JS45. Annual Meeting, American Society for Microbiology.
  98. Wallace, W.H., D.E. Miller, and J.C. Spain, 1993. Biodegradation of chlorobenzene by indigenous microorganisms and Pseudomonas sp. JS150 in an aboveground fixed-film bioreactor. Annual Meeting, Society of Environmental Toxicology and Chemistry.
  99. Nishino, S.F., and J.C. Spain, 1993. Isolation of nitrobenzene-degrading bacteria from contaminated sites. Annual Meeting, Society of Environmental Toxicology and Chemistry.
  100. Nishino, S.F., and Spain, J.C., 1993. Initial steps in the mineralization of nitrobenzene by a Pseudomonas pseudoalcaligenes. Annual Meeting, American Society for Microbiology.
  101. Vogel, C.M., G. Klecka, A. Thomas, and J.C. Spain, 1993. Biodegradation of chloroaromatic compounds in aboveground bioreactors. Annual Meeting American Chemical Society.
  102. Jain, R.K., J.H. Driesbach, and J.C. Spain, 1993. Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp. Annual Meeting, Society of Environmental Toxicology and Chemistry.
  103. Jain, R.K., Suen, W.C., and Spain, J.C., 1993. Cloning and expression of 2,4-dinitrotoluene degrading genes from a Pseudomonas sp. R34 in Escherichia coli. Annual Meeting, American Society for Microbiology.
  104. Reardon, K.F., and Spain, J.C., 1993. Immobilized cell bioreactor for 2,4-dinitrotoluene degradation. American Chemical Society National Meeting.
  105. Pettigrew, C.A., J.C. Spain, S.F. Nishino, and W. Wallace. 1993. Biodegradation of groundwater contaminants by indigenous and specific chlorobenzene-degrading bacteria. Bioremediation Symposium, Soil Science Society of America.
  106. Reardon, K.F., and Spain, J.C., 1993. Immobilized cell bioreactor for 2,4-dinitrotoluene degradation. Second International Symposium on In Situ and On-Site Bioreclamation.
  107. Haigler, B.E., and Spain, J.C., 1993. Degradation of 4-nitrotoluene and 4-nitrobenzoate by a Pseudomonas species. Annual Meeting, American Society for Microbiology.
  108. Suen, W.C., and Spain, J.C., 1993. Nucleotide sequence and overexpression of 2,4,5-trihydroxytoluene oxygenase gene from Pseudomonas sp. strain DNT in Escherichia coli. Annual Meeting, American Society for Microbiology.
  109. Wallace, W.H., Haigler, B.E., and Spain, J.C., 1993. Bacterial degradation of 2-nitrotoluene. Annual Meeting, American Society for Microbiology.
  110. Suen, W.C., and J.C. Spain, 1992. Cloning and characterization of Pseudomonas sp. strain DNT genes for 2,4-dinitrotoluene degradation. American Chem. Soc. Ninth International Biotechnology Symposium.
  111. Spain, J.C., S.F. Nishino, and C.A. Pettigrew, 1992. Biodegradation of groundwater contaminants by indigenous organisms. Annual Meeting, Society of Environmental Toxicology and Chemistry.
  112. Spain, J.C., S.F. Nishino, and B.F. Haigler, 1992. Biodegradation of nitroaromatic compounds. Bat-Sheva de Rothchild Foundation Symposium on Use of Natural and Genetically Engineered Microorganisms to Combat Pollution. Tel Aviv University, Israel.
  113. Haigler, B.E., S.F. Nishino and J.C. Spain, 1992. Bacterial degradation of 2,4-dinitrotoluene. National Meeting, American Society of Microbiology.
  114. Nishino, S.F., and J.C. Spain, 1992. Initial steps in the bacterial degradation of 1,3-dinitrobenzene. National Meeting, American Society of Microbiology.
  115. Robertson, J.B., and J.C. Spain, 1991. Oxidation of nitrotoluenes by toluene dioxygenase. Annual Meeting, Society for Environmental Toxicology and Chemistry.
  116. Haigler, B.E., S.F. Nishino, J.C. Spain, and R.J. Spanggord, 1991. Metabolic pathway for bacterial degradation of 2,4-dinitrotoluene. Gordon Research Conference on Applied and Environmental Microbiology.
  117. Nishino, S.F., R.J. Spanggord, and J.C. Spain. 1991. Initial steps in the bacterial degradation of 2,4-dinitrotoluene. National Meeting American Society for Microbiology.
  118. Haigler, B.F. and J.C. Spain. 1991. Degradation of nitrobenzene by bacteria containing toluene degradative pathways. National Meeting American Society for Microbiology.
  119. Pettigrew, C.A., J.C. Spain, and C.M. Vogel. 1991. Biological treatment of groundwater contaminated with mixtures of aromatic compounds. International Symposium on In Situ and On Site Bioreclamation.
  120. Henley, M.K., and J.C. Spain. 1991. Thermospray LC-MS of microbiologically important acids. Pittsburg Conference American Chemical Society.
  121. Spain, J.C. and S.F. Nishino. 1990. Metabolite accumulation serves as intercellular signal for induction of enzymes of p-nitrophenol degradation pathway. ASM International Conference on Multicellular Behavior of Bacteria. Woods Hole MA.
  122. Pettigrew, C.A., B.E. Haigler, and J.C. Spain. 1990. Simultaneous biodegradation of chlorobenzene and toluene by a Pseudomonas strain. Annual Meeting, American Society for Microbiology.
  123. Nishino, S.F. and J.C. Spain. 1990. Adaptation to biodegradation of p-nitrophenol depends on accumulation of a metabolite. Annual Meeting, American Society for Microbiology.
  124. Haigler, B.E., C.A. Pettigrew, and J.C. Spain. 1990. Multiple pathways for the biodegradation of substituted benzenes in a single strain of Pseudomonas. Annual Meeting, American Society for Microbiology.

Patents

  1. L.J. Nadeau, H. Luckarift, and J.C. Spain. 2011. Biocatalytic process for the production of ortho-aminophenols from chloramphenicol and analogs. US Patent No. 11,602,435.
  2. Spain, J.C., L.J. Nadeau, and Z. He 2004. Biological process for the production of ortho-aminophenols from nitroaromatic compounds. US Patent No. 6,797,497. September 28, 2004.
  3. He, Z., and J. C. Spain. 2002. Preparation of 2-aminomuconate from aminophenol by coupled enzymatic dioxygenation and dehydroxylation. US Patent No. 6,432,683. Aug. 13, 2002.
  4. Spain, J.C., S.F. Nishino, and U. Lendenmann. Process for the biodegradation of dinitrotoluene. US Patent No. 6,248,580 B1. June 19, 2001.
  5. Nadeau, L.J., J.C. Spain, and V. Kadiyala. 2005. Biological process for the conversion of nitroarenes to ortho-aminophenols using recombinant E. coli strains. US Patent No. 7,364,881. April 29, 2008.
  6. Tan, L-S.,R. Kannan, J.C. Spain and L. Nadeau. 2006. Enzymatically Produced o-Aminophenol Containing AB-Monomer for Heterocyclic Rigid-Rod Polymer Synthesis. 7,495,106. Feb. 24, 2009.